simple protocol for plant regeneration of lilium ledebourii using transverse thin cell layer

نویسندگان

masoud mirmasoumi

pejman azadi

ali sharafi

valentine ntui otang

masahiro mii

چکیده

transverse thin cell layer sections excised from in vitro scales of lilium ledebourii were cultured on murashige and skoog (ms) medium supplemented with various plant growth regulators (pgrs) at different concentrations. although, bulblets were produced on pgrfree ms medium during organogenesis, addition of 0.25 mg l-1 6-benzyladenine or 5.0 mg l-1 indole-3-acetic acid to the medium increased the organogenesis response and produced 4.4 bulblets per explants. two months later, the bulblets were transferred to ms pgrs-free medium. bulblets were successfully transplanted to the soil after a cold treatment of 8 weeks,with a survival rate of 85%.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Simple protocol for plant regeneration of Lilium ledebourii using transverse thin cell layer

Transverse thin cell layer sections excised from in vitro scales of Lilium ledebourii were cultured on Murashige and Skoog (MS) medium supplemented with various plant growth regulators (PGRs) at different concentrations. Although, bulblets were produced on PGRfree MS medium during organogenesis, addition of 0.25 mg l-1 6-benzyladenine or 5.0 mg l-1 indole-3-acetic acid to the medium increased t...

متن کامل

Plant Regeneration from Protoplasts of Lilium ledebourii (Baker) Boiss

For half a century, the limitations of obtaining cross-combinations in lilies because of the incompatibility and incongruity between different varieties have been known. Somatic hybridization is one of the most powerful tools for achieving distant interspecific hybrids. For this purpose, protoplast preparation is a first and important step in efficient system for the regeneration of plants from...

متن کامل

plant regeneration from protoplasts of lilium ledebourii (baker) boiss

for half a century, the limitations of obtaining cross-combinations in lilies because of the incompatibility and incongruity between different varieties have been known. somatic hybridization is one of the most powerful tools for achieving distant interspecific hybrids. for this purpose, protoplast preparation is a first and important step in efficient system for the regeneration of plants from...

متن کامل

Modelling of the spatial distribution of the rare plant Lilium ledebourii

The aim of this study was modelling the spatial distribution ofLilium ledebourii (Baker) Boiss. based on ecological characteristics, in order to predict potential habitats for conservation of a rare plant. Knowledge of the spatial distributions of rare and threatened species and the underlying ecological factors plays an important role in regional conservation assessments and development planni...

متن کامل

Effects of liquid, temporary immersion bioreactor and solid culture systems on micropropagation of Lilium ledebourii via bulblet microscales— An endangered valuable plant with ornamental potential

Lilium ledebourii (Baker) Boiss. (Liliaceae) is a critically endangered lily species native tonorthern Iran, where it is protected by law. In order to develop a cost effective method for largescalepropagation, the effects of three culture systems (solid, liquid and temporary immersion)and two types of cytokinins [6-Benzyladenine (BA) and Thidiazuron (TDZ)] were studied onthe in vitro plant rege...

متن کامل

A Simple Apparatus for Thin-layer Chromatography.

Thin-layer chromatography was introduced by Stahl (1956) and has advantages over paper chromatography in speed, resolution, and smaller sample size. Also, the physical nature of the thin layer allows its treatment, where necessary, with strong acids and alkalis. It has been found possible to construct a satisfactory, cheap, and simple apparatus for the preparation of thin layers on glass carrie...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
progress in biological sciences

ناشر: university of tehran

ISSN 1016-1058

دوره 3

شماره 2 2013

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023